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. 2020 Apr 28;10:7161. doi: 10.1038/s41598-020-64132-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Volume-rendered images of the spermatheca of a young adult female (a–h) and of an older adult female, where the calyx is not rendered (i). Left-lateral section (a,d,e), details of the structures inside the calyx and proximal spermathecal duct (e–h), internal view proximal at the level where the calyx connects (f), a detailed view showing the bigger foramen and the closing muscles (g), and pierced wall that separates the proximal duct chamber from the interior of the calyx - the blue arrow points to the hole where the valve plug inserts (h).