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. 2020 Apr 28;40(4):BSR20200905. doi: 10.1042/BSR20200905

Figure 2. CQ and BafA treatment acutely increase intralysosomal pH.

Figure 2

A549 cells were treated with 1:1000 DMSO (vehicle), 50 µM CQ, or 200 nM BafA, for 1, 2, or 6 h. Culture medium was additionally supplemented with LysoTracker Red DND-99 for 1 h prior to fixing. All samples were then analysed via confocal microscopy. Each panel represents a set of cells labelled with DAPI (blue) and LysoTracker Red DND-99 (red). The scale bar represents 10 µm. Results are representative of three independent experiments.