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. 2020 Apr 28;40(4):BSR20194118. doi: 10.1042/BSR20194118

Figure 5. GGA induces priming of the NLRP3 inflammasome through TLR4.

Figure 5

Total RNA was extracted at the indicated time points after 20 µM GGA treatment to estimate the levels of NLRP3 (A) and IL1B (B) mRNAs by RT-qPCR with the internal reference control 28S rRNA. (C) The level of NLRP3 mRNA was measured by RT-qPCR at 5 h after 20 µM GGA treatment in the absence (−) or presence of 50 µM oleic acid (OA+GGA), 100 µM α-tocopherol (α-Toc+GGA), or 5 µM VIPER (VIPER+GGA). Values are the means ± SD (n=3). ** indicates statistical significance (P<0.01) compared with controls as determined by the Student’s t test.