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. 2020 Apr 29;21:23. doi: 10.1186/s12865-020-00354-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — In vitro-expanded CD8⁺CD28⁻ T cells possess durable suppressive activity at different time points in vitro. The in vitro-expanded CD8⁺CD28⁻ T cells (2.5 × 10⁴/well), which had been primed with B-APCs during the expansion culture, were tested for the ability to suppress proliferation of autologous CFSE-labeled CD4⁺ T cells (5 × 10⁴/well) stimulated with allogenic APCs (5 × 10⁴/well) in vitro for 7 days and 11 days. The top and bottom displayed in the histogram were the data expressed as percentage of CD4⁺ T cell proliferation in the B-APCs (top) or the I-APCs (bottom) stimulating group measured by CFSE dilution from a representative experiment after 7 days (a) or 11 days (c) of coculture. Statistical analysis displayed in bar graphs showed the suppression percentage of CD4⁺ T cells proliferation by the in vitro-expanded CD8⁺CD28⁻ T cells in the B-APCs or the I-APCs stimulating group after 7 days (b) or 11 days (d) of coculture (n = 3). *** P<0.001