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. 2020 Apr 28;18:65. doi: 10.1186/s12951-020-00615-4

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — SP-AH-mediated delivery of MIR376B into mice blocked autophagy in xenografted breast tumors. aMIR376B levels (qPCR) in xenografted MDA tumors following tail vein i.v. injection of PBS or SP-AH/MIR376B (mean ± SD, n = 6 tumors per treatment condition, ***p < 0.01). bBECN1 mRNA levels (qPCR) in xenografted MDA tumors (mean ± SD, n = 8 tumors per treatment condition, ***p < 0.01). cATG4C mRNA levels (qPCR) in xenografted MDA tumors (mean ± SD, n = 8 tumors per treatment condition, *p < 0.05). d Immunostaining of tissue sections of tumors from PBS- or SP-AH/MIR376B-injected mice using anti-BECN1, anti-ATG4C and anti-LC3 antibodies. e Immunoblot analysis of BECN1, ATG4C and LC3 in the tumor extracts from PBS- or SP-AH/MIR376B-injected mice. ACTB was used as loading control. BECN1/ACTB, ATG4C/ACTB and LC3-II/I band intensity ratios were marked. Scale bar: 25 µm