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. 2020 Apr 28;40(4):BSR20192920. doi: 10.1042/BSR20192920

Figure 6. TPL reversed the function of IL4 overexpression promoting cell apoptosis.

Figure 6

(A) IL4 protein and mRNA levels were detected by Western blot and RT-PCR assays. (B) The viability of podocytes by CCK8 assay in cell with IL4. (C) The apoptosis level of podocytes by flow cytometry assay in cell with IL4. (D) The protein levels of nephrin and podocin, and phosphorylation level of Stat6 by Western blotting analysis. GAPDH was used as a load control. Data are presented as the mean ± standard deviation. aP<0.05 versus vector group, and bP<0.05 versus IL4 group.