Table 1.

Summary of parameters used with each substrate tested in the zymography.

Electrophoresis
Gel	I	II
Constituents 1	4–12% mini polyacrylamide gel 1
Temperature of the run	room temperature
Total protein loaded per well	10 µg
Running buffer	Tris-glycine pH 8.3
Electric field applied	100 V constant and ~350 mA
Run time	3 h
Hydrolytic activity assay
Substrate solution and concentration	Maltose 56 mM	Sucrose 56 mM
Incubation time	1 h	1 h
Incubation temperature	37 °C
Assay reagent 2 Incubation time (reaction temperature)	1 h (37 °C)	5 h (37 °C)
Rinse the gel with deionized water	3 ×
Gel storage for further analysis	4 °C

References: ¹ Novex^TM 4–12% Tris-glycine mini polyacrylamide gel; ² Glucose (GO) Assay Kit (Sigma GAGO-20).