Table 2.
Hydrolases identified by nano-liquid chromatography coupled to mass spectrometry in the slices cut from gels incubated with maltose and sucrose. Quantitative value and coverage of enzymes in the analyzed sample was determined using Scaffold software package (version 4.3.2, Proteome Software Inc., Portland, OR, USA). The % coverage refers to the percentage of the protein sequence covered by identified peptides. Protein identifications were accepted if they could be established at greater than 99.0% probability with a 1% false discovery rate and containing at least 5 identified peptides.
| Enzyme | Uniprot Accesion Number | Molecular Mass (kDa) | Maltose Substrate | Sucrose Substrate | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Mouse 1 | Mouse 2 | Mouse 1 | Mouse 2 | |||||||
| Quantitative Value (Normalized Total Spectra) | Coverage % | Quantitative Value (Normalized Total Spectra) | Coverage % | Quantitative Value (Normalized Total Spectra) | Coverage % | Quantitative Value (Normalized Total Spectra) | Coverage % | |||
| SI (sucrase-isomaltase) | F8VQM5 | 209 | 104 | 23 | 42 | 3 | 100 | 35 | 81 | 6 |
| MGAM (maltase-glucoamylase) | B5THE2 | 209 | 56 | 15 | 94 | 6 | 83 | 31 | 38 | 4 |
| ANPEP (aminopeptidase-N) | P97449 | 110 | 25 | 11 | 42 | 2 | 49 | 39 | 49 | 5 |
| ENPEP (Glutamyl Aminopeptidase) | P16406 | 108 | 9 | 8 | - | - | 12 | 21 | - | - |
| DPP4 (Dipeptidyl peptidase 4) | P28843 | 87 | 3 | 4 | - | - | 7 | 16 | - | - |
| MEP1B (Meprin A Subunit Beta) | Q61847 | 80 | 3 | 10 | - | - | 5 | 12 | - | - |
| All other non-hydrolase proteins (details in Supplementary Table S1) |
194 | 147 | 210 | 211 | ||||||
| Hydrolases as % of total proteins | 51% | 55% | 55% | 44% | ||||||
| α-glucosidases as % of total hydrolases | 80% | 76% | 71% | 71% | ||||||