FIGURE 2.

Human PCa Exo^Hypoxic promotes MMP2 and MMP9 expression in selective organs in male athymic nude mice. A,B, Male athymic nude mice were injected with PBS, Exo^Normoxic or Exo^Hypoxic (10 μg each, IP) on alternate days for 4 weeks. At the end, mice were killed and mentioned organs were collected, processed, and analyzed for MMP2 and MMP9 expression by IHC as described in methods. Immunoreactivity for MMP2 and MMP9 was scored as 0+ (no staining), 1+ (weak staining), 2+ (moderate staining), 3+ (strong staining), 4+ (very strong staining). Data shown in the bar diagram represent mean ± SEM of immunoreactivity scores for ten randomly selected fields from 4 to 5 samples for each group. Representative images are shown at ×400. *P ≤ .05. Exo^Hypoxic, exosomes secreted under hypoxic condition; Exo^Normoxic, Exosomes secreted under normoxic condition; IHC, immunohistochemistry; IP, intraperitoneal; MMP, matrix metalloproteinases; PBS, phosphate buffered saline; PCa, prostate cancer [Color figure can be viewed at wileyonlinelibrary.com]