FIGURE 3.

Human PCa Exo^Hypoxic promote matrix remodeling in selective organs in male athymic nude mice. A,B, Male athymic nude mice were injected with PBS, Exo^Normoxic or Exo^Hypoxic (10 μg each, IP) on alternate days for 4 weeks. At the end, mice were killed and mentioned organs were collected, processed, and analyzed for fibronectin and collagen expression by IHC. Immunoreactivity for fibronectin and collagen was scored as 0+ (no staining), 1+ (weak staining), 2+ (moderate staining), 3+ (strong staining), 4+ (very strong staining). Data shown in the bar diagram represent mean ± SEM of immunoreactivity scores for ten randomly selected fields from 4 to 5 samples for each group. Representative images are shown at ×400. *P ≤ .05. Exo^Hypoxic, exosomes secreted under hypoxic condition; Exo^Normoxic, Exosomes secreted under normoxic condition; IHC, immunohistochemistry; IP, intraperitoneal; MMP, matrix metalloproteinases; PBS, phosphate buffered saline; PCa, prostate cancer [Color figure can be viewed at wileyonlinelibrary.com]