FIGURE 4.

Effect of PCa Exo^Hypoxic treatment on CD11b+ cells in male athymic nude mice. Male athymic nude mice were injected with PBS, Exo^Normoxic or Exo^Hypoxic (10 μg each, IP) on alternate days for 4 weeks. At the end, mice were killed and mentioned organs were collected, processed, and analyzed for CD11b+ cells by IHC and presented as % positive cells. Data shown in the bar diagram represent mean ± SEM of immunoreactivity scores for ten randomly selected fields from 4 to 5 samples for each group. Representative images are shown at ×400. *P ≤ .05. Exo^Hypoxic, exosomes secreted under hypoxic condition; Exo^Normoxic, Exosomes secreted under normoxic condition; IHC, immunohistochemistry; IP, intraperitoneal; MMP, matrix metalloproteinases; PBS, phosphate buffered saline; PCa, prostate cancer [Color figure can be viewed at wileyonlinelibrary.com]