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. 2020 Apr 29;40(18):3533–3548. doi: 10.1523/JNEUROSCI.2378-19.2020

Figure 1.

Figure 1.

MB output is not required for DA release on coincident MB stimulation. A, Inhibiting MB output impairs recall of olfactory memory. One-way ANOVA and Bonferroni post hoc tests indicate significant impairment in memory recall in MB > shits flies at a restrictive temperature (32°C) (F(2,15) = 71.05, p < 0.001) but not at a permissive temperature (22°C) (F(2,15) = 2.854, p = 0.09). **p < 0.01. Not significant (p > 0.05). N = 6 for all data. B, C, Blocking SV exocytosis from the MBs does not affect DA release. spH fluorescence was measured at TH-DA terminals innervating the α3/α′3 compartments of the MB vertical lobes in MB-LexA>LexAop-shits (B) and c747-GAL4>UAS-shits brains (C). Left, Typical pseudocolor images 5 s before and 30 s after coincident AL + NMDA activation. Middle, Time course of fluorescent changes. Arrows represent AL stimulation. Right, Summary graphs. Two-way ANOVA indicates no significant differences in spH fluorescence between restrictive (32°C) and permissive (22°C) temperatures in both MB-LexA>LexAop-shits (F(3,64) = 2.854, p = 0.5716, N = 9 for all data) and c747-GAL4>UAS-shits (F(3,68) = 2.854, p = 0.01, N = 9 for 22°C, N = 10 for 32°C). Scale bars, 20 μm.