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. 2020 Apr 29;40(18):3533–3548. doi: 10.1523/JNEUROSCI.2378-19.2020

Figure 3.

Figure 3.

dHO in the MBs is required LTE and olfactory learning. A, An HO inhibitor, CrMP, prevents LTE induced by AL+ NMDA stimulation. One-way ANOVA and Bonferroni post hoc tests indicate significant changes in AL-evoked Ca2+ responses in the MB after AL + NMDA stimulation in control conditions (F(2,15) = 5.836, p = 0.013, N = 6) but not in 10 μm CrMP-treated conditions (F(2,18)= 0.339, p = 0.717, N = 7). B, dHO antibody labels the MB lobes and midline cells in the Drosophila brain. Fas II antibody staining is included to identify subsets of the MB lobes. dHO signals are reduced in dHOΔ hemizygotes (dHOΔ/+), demonstrating the specificity of the antibody. C, Knockdown of dHO in the MBs impairs LTE induced by AL + NMDA stimulation. One-way ANOVA and Bonferroni post hoc tests indicate significant LTE in the MB after AL + NMDA stimulation in control (RU–) conditions (left: F(2,18) = 9.630, p = 0.001, N =7), but not in dHO-knockdown (RU+) conditions (right: F(2,21) = 0.444, p = 0.647, N = 8). D, Knocking down dHO in the MBs impairs olfactory learning. **p < 0.01 (Student's t test). N = 7 for all data. E, Naive responses to odors and electrical shock are not affected by knocking down dHO in the MBs (p > 0.05, Student's t test). N = 8 for all experiments.