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. 2020 Apr 29;40(18):3533–3548. doi: 10.1523/JNEUROSCI.2378-19.2020

Figure 5.

Figure 5.

CO evokes SV exocytosis from DA terminals. A, CO induces DA release. CO-saturated saline was puffed onto the vertical lobes of the MB during the indicated time period (middle, gray-shaded area). CO-induced spH responses were obtained by subtracting spH fluorescent changes induced by control N2-satulated saline from fluorescence changes induced by CO-saturated saline. One-way ANOVA and post hoc tests indicate significant changes in spH fluorescence on CO application (F(3,44)= 14.994, p < 0.001). N = 12 for all data. **p < 0.01 (Bonferroni post hoc tests). B, CORM-3 induces DA release; 20 μm CORM-3 (dissolved in 0.2% DMSO) was bath-applied, and spH fluorescence was measured at DA terminals at the α3/α′3 compartments of the MB vertical lobes. CORM-3-induced spH responses were obtained by subtracting fluorescent changes induced by control 0.2% DMSO saline from fluorescence changes induced by CORM-3. **p < 0.01 (Student's t test), comparing spH responses before (0-30s) and after application of CO (270-300s). N = 8 for all data. C, Arachidonic acid (AA) does not induce DA release; 200 μm AA (dissolved in 0.4% ethanol) was bath-applied. AA-induced spH responses were obtained by subtracting fluorescence changes induced by 0.4% ethanol saline from fluorescence changes induced by AA. Not significant (p > 0.05, Mann-Whitney test). N = 16 for all data. D, Effects of 2-arachidonylglycerol (2-AG) on DA release; 200 μm AG (dissolved in 0.2% DMSO) was bath-applied. AG-induced spH responses were obtained by subtracting fluorescence changes induced by 0.2% DMSO saline from fluorescence changes induced by AG. Not significant (p > 0.05, Student's t test). N = 12 for all data. E, The CO scavenger, hemoCD, prevents SV exocytosis from DA terminals. AL + NMDA-dependent changes in spH fluorescence were measured in the presence and absence of 50 μm hemoCD. Two-way ANOVA indicates significant decreases in spH fluorescence due to hemoCD treatment (F(3,56) = 2.845, p = 0.0458, N = 8 for all data). **p < 0.01 (Bonferroni post hoc tests).