Figure 8.
SV exocytosis from DA terminals induced by coincident AL + NMDA stimulation requires RyR. A, An IP3R inhibitor does not affect DA release; 100 μm 2-aminoethoxydiphenylborane (2-APB) was dissolved in 0.1% DMSO saline. Two-way ANOVA indicates no significant differences in spH fluorescence due to 2-APB treatment. N = 10 for 2-APB treatment and N = 9 for mock control. B, The RyR inhibitor, dantrolene, inhibits DA release; 10 μm dantrolene was dissolved in 0.1% DMSO saline. Two-way ANOVA indicates significant effects of drug treatment on spH fluorescence (F(1,48) = 6.781, p = 0.0122, N = 8 for all data). *p < 0.05 compared with mock-treated samples assayed (Bonferroni post hoc tests). C, Application of a RyR agonist induces DA release. 4-Chloro-3-methylphenol (4C3MP) was dissolved at 1 mm in 0.2% ethanol saline containing 1 μm TTX and applied for the indicated period of time (yellow). Two-way ANOVA indicates significant effects of drug treatment on spH fluorescence (F(1,28) = 5.466, p = 0.027). N = 8 for all data. *p < 0.05 (Bonferroni post hoc tests).