Table 4.
IC50 Values for AES-135 in Several Monolayer and 3D Human-Derived PDAC Cell Lines (n = 3–5)
| monolayer PDAC cells | ||
|---|---|---|
| cell line | IC50 (μM) | |
| Pa02C | 4.6 | |
| Pa03C | 3.4 | |
| 10.05 | 3.9 | |
| KPC | 1.3 | |
| 3D PDAC cells | ||
| cell line | scan type | IC50 (μM) |
| Pa03C | areaa | 1.22 |
| Pa03C + CAFs | area | 1.41 |
| CAF coculture | area | 7.80 |
| Pa03C | intensityb | 1.33 |
| Pa03C + CAFs | intensity | 1.56 |
| CAF coculture | intensity | 4.50 |
| 10.05 | area | 0.97 |
| 10.05 + CAFs | area | 0.94 |
| CAF coculture | area | 4.70 |
| 10.05 | intensity | 0.60 |
| 10.05 + CAFs | intensity | 0.50 |
| CAF coculture | intensity | 3.40 |
a
Area: the μ2 of objects that exceed a minimum tomato red intensity threshold in the well.
b
The sum of all intensity values for pixels marked as χ2 objects (i.e., total red OR green fluorescence in the well, after background removal).