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. 2020 Apr 29;15(4):e0231762. doi: 10.1371/journal.pone.0231762

Fig 3. Colony formation assay.

Fig 3

(A) Abrams, D-17, and HMPOS canine OSA cell lines treated with vehicle control (DMSO), 33% of the respective determined IC50 dose of vismodegib (10 μM for Abrams and HMPOS; 15 μM for D-17), 66% of the respective determined IC50 dose of vismodegib (20 μM for Abrams and HMPOS; 30 μM for D-17), and the full determined IC50 dose of vismodegib (30 μM for Abrams and HMPOS; 45 μM for D-17). Abrams, D-17, and HMPOS cell lines demonstrated decreased colony formation following treatment with vismodegib at the respectively determined IC50 value compared to vehicle control treatment. (B) Quantitation of colony formation assays reflected by survival fraction. * p < 0.05; **p < 0.01.