Table 1. In Situ hybridization scoring of osteosarcoma samples.

Tumor Sample	Smo	Ptch1	Gli1	Gli2	Ihh
1	+	++	++	+	-
2	+++	-	+	-	+
3	-	-	-	+	-
4	+	+	-	+	-
5	+++	+++	+++	++	+
6	+++	+	++	++	-
7	+	++	+	+	+
8	++	+	-	-	-
9	++++	+++	++	++	++
10	++++	+++	-	-	-
11	++++	+++	+++	+++	+++

No staining; + <25% cells staining positive; ++ 25–50% staining; +++ 50–75% staining; ++++ >75% staining.