KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal antibody anti-SRPKl | BD Biosciences | 611072 |
| Mouse monoclonal antibody anti-SRPK2 | BD Biosciences | 611118 |
| Mouse monoclonal antibody anti-Protamine 1 | Briar Patch Biosciences | Hup1N |
| Mouse monoclonal antibody anti-HIRA | Active Motif | 39557 |
| Mouse monoclonal antibody anti-SC35 | Gui et al., 1994a | N/A |
| Goat polyclonal antibody anti-Protamine 1 | Santa Cruz | SC-23107 |
| Rabbit polyclonal antibody anti-Flag | Sigma-Aldrich | F7425 |
| Rabbit polyclonal antibody anti-HA | Cell Signaling | 3724 |
| Rabbit polyclonal antibody anti-SRPK1 | ABclonal | A5854 |
| Rabbit polyclonal anti-P1-pSer9 | This paper | N/A |
| Rabbit polyclonal anti-P1-pSer11 | This paper | N/A |
| Rabbit polyclonal anti-P1-pSer13 | This paper | N/A |
| Rabbit polyclonal anti-P1-pSer43 | This paper | N/A |
| Bacterial and Virus Strains | ||
| BL21 (DE3) competent E.coli | BioLabs | C2527 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Gonadotropin Pregnant Mare Serum (PMSG) | Millipore | 367222 |
| Chorionic Gonadotropin Human (hCG) | Millipore | C1063 |
| EmbryoMax HTF | Millipore | MR-070-D |
| EmbryoMax M2 | Millipore | MR-015-D |
| EmbryoMax M2 with Hyaluronidase | Millipore | MR-051-F |
| EmbryoMax Acidic Tyrode’s Solution | Millipore | MR-004-D |
| EmbryoMax KSOM | Millipore | MR-121-D |
| Aniline Blue solution | Sigma-Aldrich | B8563 |
| Insulin-transferrin-sodium selenite media supplement | Sigma-Aldrich | I1884 |
| Recombinant Human EGF | R&D Systems | 236-EG |
| Chemically synthesized peptides, see Table S1 | This paper | See Table S1 |
| EnGen™ Cas9 NLS | BioLabs | M0646T |
| Critical Commercial Assays | ||
| Nextera DNA Library Prep Kit | Illumina | 15028212 |
| SPRI Beads | Beckman Coulter | B23319 |
| DNA Clean & Concentrator −5 kit | Zymo Research | D4013 |
| Amicon Ultra-0.5 Centrifugal Filter devices | Millipore | UFC500324 |
| Atto 488 protein labeling kit | Sigma-Aldrich | 38371 |
| ULYSIS Nucleic acid labeling kits | Molecular Probes | U21654 |
| Micro Bio-spin columns P-30, Tris | Bio-Rad | 732–6250 |
| Mouse Testis genomic DNA | ZYAGEN | MG-401 |
| HiTrap Columns, CM Sepharose FF (1 mL) | GE Healthcare | 95056–086 |
| HiTrap Columns, DEAE Sepharose FF (1 mL) | GE Healthcare | 95056–084 |
| Dynabeads™ Protein G | ThermoFisher | 10009D |
| Deposited Data | ||
| ATAC-seq data | This paper | GSE136403 |
| Experimental Models: Cell Lines | ||
| Human HEK293T | ATCC | CRL-3216 |
| Oligonucleotides | ||
| ON-TARGET plus Mouse Srpk1 siRNA | Dharmacon | J-061647–08 |
| crRNAs, see Table S2 | This paper | N/A |
| tracrRNA, see Table S2 | This paper | N/A |
| Donor DNA, see Table S2 | This paper | N/A |
| 25, 50, 100 nt Cy3 labelled sense oligos, Table S2 | This paper | N/A |
| 25, 50, 100 nt Antisense oligos, see Table S2 | This paper | N/A |
| Recombinant DNA | ||
| pRSETb-His-SRPK1 | Aubol et al., 2012 | N/A |
| pRSETb-His-SRPK1 (KD) | Aubol et al., 2012 | N/A |
| pET23-hNpm2 | Platonova et al., 2011 | N/A |
| pcDNA3-HA | This paper | N/A |
| pcDNA3-HA-hNpm2 | This paper | N/A |
| pcDNA3-HA-HIRA | Hall et al., 2001 | N/A |
| Software and Algorithms | ||
| ImageJ | NIH | https://imagej.nih.sov/ij/ |