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. 2020 Apr 29;6(18):eaaz6579. doi: 10.1126/sciadv.aaz6579

Fig. 5. Efficacy of IFN-γ backpacks for reducing metastasis and tumor burden of 4T1 mammary carcinomas.

Fig. 5

(A) In vivo bioluminescence imaging of metastatic colony formation in the chest cavities of mice burdened with 4T1-Luc cells 32 days after inoculation (primary tumor outside of view). Five representative images per treatment group are shown. (B) Average radiance from bioluminescence in the chest cavities of the mice in (A) (n = 9). (C) Representative histological section of a 4T1 tumor treated with macrophages carrying IFN-γ backpacks. Dotted line separates regions of cleared (top) and intact tumorous tissue (bottom). (D) Relative proportion of tumor-infiltrating dendritic cells (TIDCs) in solid 4T1 tumors revealed through tumor-associated immune cell phenotyping (determined by CD45+, SYTOX−, and CD11c+; n = 5). (E) Weight changes of mice burdened with 4T1-Luc tumors in different groups (n = 9). (F) Growth kinetics of tumors in the groups shown in (E). Black arrows indicate days of therapeutic injections. (G) Survival of mice in (E). Statistical significance was determined via a log-rank test. *P < 0.05; **P < 0.01; ***p < 0.001.