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. 2020 Apr 14;9:e54575. doi: 10.7554/eLife.54575

Figure 2. The 5'-UTR-IRES domain I engages ribosomal proteins RACK1 and uS3.

(A) Overview of the 40S–5'-UTR-IRES–eIF3 map, with 40S and eIF3 depicted gray and 5'-UTR-IRES in blue. (B) Detailed view of the cryo-EM density of the 40S–5'-UTR-IRES–eIF3 map centered around 5'-UTR-IRES domain I (DI). Ribosomal proteins are colored brown, 18S rRNA yellow and 5'-UTR-IRES blue. Contacts between 5'-UTR-IRES domain I and ribosomal proteins RACK1 (C) and uS3 (D) could be defined thanks to well-resolved local cryo-EM densities.

Figure 2.

Figure 2—figure supplement 1. Structurally derived secondary structure diagram for the 5'-UTR-IRES.

Figure 2—figure supplement 1.

Secondary structure diagram for the 5'-UTR-CrPV IRES derived from the cryo-EM structure.