FIG 7.

Reorganization of tet(X4)-bearing plasmids resolved by S1-PFGE and the underlying molecular mechanisms. (a) S1-PFGE of donor strains and the corresponding transconjugants with plasmid reorganizations (M, molecular weight markers); (b to f) schematic diagrams depicting the generation process of five cointegrate plasmids mediated by homologous regions or IS26. Red arrows indicate the tet(X4) gene, and green arrows represent the genes involved in plasmid reorganizations. Target site duplications are shown with purple rectangles. The plasmid reorganization of samples RF15-1 and TRF15-1 was not resolved successfully here.