FIG 1.

(A) Growth curves for Rothia mucilaginosa ATCC 25296 incubated under aerobic conditions in liquid M9 medium, supplemented with different carbon sources (x axis, hours; y axis, optical density [OD₆₀₀]). (B) Colorimetric absorbance at 500 nm (y axis, A₅₀₀) capturing catecholate derivatives in liquid R. mucilaginosa (Rmuc) and R. dentocariosa M567 (Rdent) growth cultures (x axis) using Arnow’s assay. R. mucilaginosa was grown in M9 medium supplemented with 100 mM glycerol (Glyc_Rmuc), 100 mM lactate (Lac_Rmuc), and 100 mM sucrose (Suc_Rmuc). R. dentocariosa was grown in 100 mM glycerol to see if glycerol induced siderophore production as seen for R. mucilaginosa cultures.