. 2020 Apr 29;9(5):e1133. doi: 10.1002/cti2.1133

Table 2.

Antibodies used for human mass cytometry staining

Target	Isotope	Clone	Company	tSNE ^a	IgG₃ gating ^b
CD3	155Gd	UCHT1	BioLegend
CD19	142Nd ^c	HIB19	BD Biosciences	✓
CD20	147Sm ^c	2H7	BioLegend	✓	✓
CD21	152Sm ^c	BU32	BioLegend	✓	✓
CD23	144Nd	M‐L23.4	Miltenyi Biotec	✓
CD24	151Eu	ML5	BioLegend	✓	✓
CD25	149Sm ^c	2A3	BD Biosciences	✓
CD27	167Er ^c	M‐T271	BioLegend	✓	✓
CD38	154Sm	HIT2	BioLegend	✓	✓
CD45 ^d	104Pd ^c	HI30	BD Biosciences
	115In ^c
	209Bi ^c
CD71	164Er	CY1G4	BioLegend	✓
CD79b	153Eu	CB3‐2	BioLegend	✓
CD80	162Dy ^c	L307.4	BD Biosciences	✓
CD86	156Gd ^c	IT2.2	BD Biosciences	✓
CD120a	158Gd	REA252	Miltenyi Biotec	✓
CD120b	160Gd	3G7A02	BioLegend	✓
CD138	150Nd ^c	DL‐101	BioLegend	✓
CD184 (CXCR4)	175Lu ^c	12G5	BD Biosciences	✓
CD185 (CXCR5)	146Nd	J252D4	BioLegend	✓
CD210 (IL‐10 receptor)	169Tm	REA239	Miltenyi Biotec	✓
CD267 (TACI)	159Gd	1A1	BioLegend	✓
CD274 (PD‐L1)	161Dy	29E.2A3	BioLegend	✓
CD360 (IL‐21 receptor)	165Ho	REA233	Miltenyi Biotec	✓
HLA‐DR	174Yb ^c	L243	BD Biosciences	✓
IgD	89Y ^c	IA6‐2	BD Biosciences
IgE	148Nd	MHE‐18	BioLegend
IgG₃	145Nd	HP6047	BioLegend
IgG₄	168Er	HP6023	BioLegend
5‐HT_2A receptor	166Er	Polyclonal	Novus Biologicals	✓
PAF receptor	163Dy	AA14 (clone21)	Cayman Chemicals	✓
S1P1	170Er	MM0045‐21L9	Novus Biologicals	✓
TNF (membrane)	141Pr ^c	Mab11	BioLegend	✓

^{^a}

Markers used to run tSNE plots.

^{^b}

Markers used for gating IgG₃ ⁺ B‐cell subsets.

^{^c}

Isotopes conjugated by the Ramaciotti Facility for Human Systems Biology, The University of Sydney, Australia.

^{^d}

CD45 was used for barcoding to allow up to three samples to be stained together.