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. Author manuscript; available in PMC: 2020 Sep 23.
Published in final edited form as: Nat Microbiol. 2020 Mar 23;5(5):679–687. doi: 10.1038/s41564-020-0691-3

Extended Data Figure 2. Characterization of kinB::Tn mutants.

Extended Data Figure 2.

a. A streak plate on X-gal plates, showing strains involved in this study and isolated transposon (Tn) insertions. csy3::lacZ is a derivative of WT PA14, and is the unmutagenized parent of kinB::Tn 1–3. b. β-galactosidase measurements of strains grown in liquid culture for the indicuated time. Measurements for the unmutagenized (csy3::lacZ) parent strain and three isolated kinB transposon mutants (kinB::Tn1–3) are shown, as well as a control PA14 culture with no lacZ insertion. c. Phage titration on lawns of the kinB::Tn1 mutant transformed with empty vector or kinB. d. Spot titration of phages JBD26 (CR2_sp17, sp20-targeted, possessing acrIF4), JBD25 (CR1_sp1 targeted) on kinB::Tn mutants and ΔCRISPR-Cas. These experiments have been replicated at least 2 times with consistent results.