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. Author manuscript; available in PMC: 2020 Aug 24.
Published in final edited form as: Nat Microbiol. 2020 Feb 24;5(5):706–714. doi: 10.1038/s41564-020-0672-6

Figure 2: Envelope stress responses protect E. coli against T6SS-mediated killing.

Figure 2:

a. Relative survival (killing by TseHWT / TseHH64A) of E. coli wild-type or knockout mutants. One-way ANOVA with Dunnett’s multiple comparisons test comparing each sample to WT; ***, p < 0.001; ns, not significant. b. Relative survival of E. coli ΔrcsB or ΔbaeR mutants with empty vector (Vec) or complementation plasmids. One-way ANOVA with Sidak’s multiple comparisons test comparing samples to their respective empty vector controls; *, p < 0.05. c. Relative survival of select E. coli mutants involved in envelope integrity (Env.) or the RcsB and BaeR regulons. One-way ANOVA with Dunnett’s multiple comparisons test comparing each sample to WT; **, p < 0.01; ***, p < 0.001; ns, not significant. WT (E. coli) data is the same as in a. and is shown again for comparison. d. RT-qPCR comparing expression of the Rcs-regulated wza gene in wild-type E. coli prey after T6SS competition assay with TseHWT or TseHH64A killer strains. Value with TseHH64A killer was set to 1 to show fold induction in response to TseH activity upon delivery by the T6SS. One-way ANOVA (with samples shown in Extended Data Fig. 3d) with Sidak’s multiple comparisons test; ***, p < 0.001. e. Relative survival (killing by V52 wild-type / ΔT6SS) of E. coli wild-type or ΔwcaJ prey overexpressing empty vector or RcsA to induce colanic acid synthesis. Killer and prey strains were mixed at a 1:1 ratio. One-way ANOVA with Tukey’s multiple comparisons test; **, p < 0.01. DL, detection limit. f. Relative survival (killing by TseHWT / TseHH64A) of E. coli mutants with empty vector or expression plasmids. One-way ANOVA with Sidak’s multiple comparisons test comparing samples to their respective empty vector controls; **, p < 0.01; ***, p < 0.001; ns, not significant. Graphs show the mean of at least three independent replicates and error bars show one standard deviation. Dots show results from individual replicates. TseHWT, V52 with all anti-bacterial effectors inactivated except TseH; TseHH64A, V52 with all anti-bacterial effectors inactivated including TseH.