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. Author manuscript; available in PMC: 2020 Aug 24.
Published in final edited form as: Nat Microbiol. 2020 Feb 24;5(5):706–714. doi: 10.1038/s41564-020-0672-6

Figure 3: V. cholerae WigR is necessary to survive T6SS self-killing, independent of immunity genes.

Figure 3:

a. Induction of TAT-tagged TseH in V52 with intact or deleted tsiH and wigR. Representative of three independent replicates. b. Relative survival (V52 TseHWT / TseHH64A killer strain) of V. cholerae V52 prey with intact or deleted tsiH and wigR. Welch’s one-way ANOVA with Games-Howell test; *, p < 0.05; ns, not significant. For ΔtsiHΔwigR compared to tsiH+wigR+ and tsiH+ΔwigR prey, p < 0.05. c. Relative survival (wild-type V52 / TseHH64A killer strain) of stationary (Stat.) and log phase V52 prey with intact (+), deleted (Δ), or plasmid complemented (p) wigR. One-way ANOVA with Sidak’s test; ***, p < 0.001. Comparing stationary and log phase, p < 0.001 for wigR+ and ΔwigR, not significant for complemented strain. d. Relative survival (wild-type V52 / ΔT6SS killer strain) of V. cholerae V52 prey lacking the tsiV1, tsiV2 and tsiV3 immunity genes (Δimm background) with intact (+), deleted (Δ), or complemented (p) wigR. Stationary (Stat.) or log phase prey were used at a 1:5 killer:prey ratio. One-way ANOVA with Sidak’s multiple comparisons test; ***, p < 0.001; **, p < 0.01. e. Relative survival (killing by A. dhakensis SSU wild-type / ΔT6SS) of V. cholerae V52 prey (ΔT6SS background) with intact (+), deleted (Δ), or complemented (p) wigR. Log phase prey were used at a 1:1 killer:prey ratio. One-way ANOVA with Sidak’s multiple comparisons test; ***, p < 0.001; **, p < 0.01. Graphs show the mean of at least three independent replicates, error bars show standard deviation. Dots show individual replicates. tsiH+ prey in a and b, and all prey in c and e are in ΔT6SS V52 background. DL, detection limit.