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. Author manuscript; available in PMC: 2020 Aug 24.
Published in final edited form as: Nat Microbiol. 2020 Feb 24;5(5):706–714. doi: 10.1038/s41564-020-0672-6

Figure 4: The protective role of WigKR supersedes VPS expression.

Figure 4:

a. Relative survival (killing by V52 wild-type / ΔT6SS) of V. cholerae V52 immunity gene mutant prey encoding or lacking vpsA (and therefore VPS). VPS expression was induced by growth of prey strains at 30 °C. One-way ANOVA with Sidak’s multiple comparisons test; ***, p < 0.001. b. Relative survival (killing by A. dhakensis SSU wild-type / ΔT6SS) of V. cholerae V52 (ΔT6SS background) encoding or lacking vpsA. VPS expression was induced by pre-growth of prey strains at 30 °C. One-way ANOVA with Sidak’s multiple comparisons test; ***, p < 0.001. c. Relative survival (killing by wild-type V52 / ΔT6SS) of log phase V. cholerae V52 prey with intact (+) or deleted (Δ) wigR and vpsA genes. As indicated, prey were grown at 37 °C or at 30 °C to induce VPS expression. One-way ANOVA with Sidak’s multiple comparisons test; **, p < 0.01; ns, not significant. DL, detection limit. Graphs show the mean of at least three independent replicates and error bars show one standard deviation. Dots show results from individual replicates.