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. 2020 Apr 29;10:7209. doi: 10.1038/s41598-020-64102-4

Figure 3.

Figure 3

α1D-AR function and plasma membrane insertion is glycosylation dependent. (A) Dynamic mass redistribution assays quantifying phenylephrine efficacy in HEK293 cells transfected with WT, N65Q, N82Q, or NQQ SNAP-α1D. Data are the mean of 8 replicates ± SEM; **p < 0.01 Emax from WT. (B) Cell surface expression of WT, N65Q, N82Q, or NQQ SNAP-α1D in fixed HEK293 cells labeled with the cell impermeable SNAP substrate, BG782 (top panel, green); nuclear stain, TO-PRO-3 was used to normalize for cell numbers (bottom panel, red). (C) Fluorescence intensity of data from B was normalized to cells expressing SNAP alone. Data are mean of 6 replicates ± SEM; One-way ANOVA with Tukey’s multiple comparisons post-hoc tests, **p < 0.01 from WT SNAP-α1D; ###p < 0.001 from empty SNAP.