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. 2020 Apr 29;10:7211. doi: 10.1038/s41598-020-64215-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Experimental setup for SVF isolation, collection of cell supernatants and processing of supernatants for miRNA analysis.Subcutaneous adipose tissue was obtained during routine outpatient liposuction procedures. The liposuction material was washed and digested with collagenase. After centrifugation of the digested fat, the cell pellet was incubated with erythrocyte lysis buffer. After another centrifugation step, the cell pellet was washed and filtered and the isolated stromal vascular fraction (SVF) was incubated for 24 h in sterile filtered medium. Conditioned medium (CM) was collected and after two centrifugation steps and filtration, it was concentrated using centrifugation-based ultrafiltration to obtain concentrated CM (cCM). Small extracellular vesicles (sEVs) were enriched from cCM by size exclusion chromatography (SEC).