Table 3.
Reactome pathways identified by pathway enrichment analysis (www.mirnet.ca) for the differentially expressed miRNAs in lipedema versus control.
| Reactomepathway enrichment analysis | #Genes | p-value |
|---|---|---|
| Oxidative Stress Induced Senescence | 7 | 0,0044 |
| Oncogene Induced Senescence | 5 | 0,0044 |
| Pre-NOTCH Transcription and Translation | 4 | 0,0064 |
| Pre-NOTCH Expression and Processing | 4 | 0,0102 |
| MicroRNA (miRNA) biogenesis | 4 | 0,0102 |
| Signaling by NOTCH | 6 | 0,0137 |
| SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription | 4 | 0,0137 |
| Signaling by Wnt | 9 | 0,0228 |
| Cellular Senescence | 7 | 0,0228 |
| Signaling by TGF-beta Receptor Complex | 5 | 0,0383 |
| Gene Expression | 17 | 0,0410 |
| Transcriptional activity of SMAD2/SMAD3:SMAD4 heterotrimer | 4 | 0,0438 |
mRNA targets for miR–16-5p, miR-29a-3p, miR-24-3p, and miR-454-p, miR–144-5p, miR-130a-3p, let-7c-5p and miR-188-5p, which significantly discriminate lipedema versus control, were identified based on experimental data supporting interactions. Reactome pathway enrichment analysis was performed by miRNet. Among the pathways likely regulated by the 8 miRNAs were the NOTCH, Wnt and the SMAD/transforming growth factor beta (TGFβ) signaling pathway, and pathways involved in oxidative stress and senescence. #Genes = number of detected genes, hypergeometric test p-value < 0.05 = significant.