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. 2020 Apr 29;10:7273. doi: 10.1038/s41598-020-64131-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Oxidative stress in the retina after fluorogold tracing. Immunodetection of 8-OHdG in retinal cross-sections analyzed 15 minutes (A), 24 hours (B) or 30 days (C) after intravitreal administration of FG in young SD rats. At 15 minutes (A’) or 24 hours (B’) there is no 8-OHdG signal. However, at 30 days there is an increase 8-OHdG signal, more evident in the areas with positive FG cells (C’). In the positive control, a P60 RCS retinal section, 8-OHdG immunodetection is observed throughout the retina most clearly in the photoreceptor outer segments (D-D’). (A”–D”) merged images. GCL: ganglion cell layer. INL: inner nuclear layer. ONL: outer nuclear layer. RPE: retinal pigment epithelium.