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. 2020 Apr 29;11:2099. doi: 10.1038/s41467-020-15872-y

Fig. 5. MALT-1 has enzymatic roles in IL-17 signaling.

Fig. 5

a–c MALT-1’s function in the nervous system requires its protease active site. a malt-1(syb296) mutants that express a catalytically inactive MALT-1 (C374A) show O2 response defects comparable to those of malt-1(null) animals. Pan-neuronal expression of malt-1 cDNA rescues this phenotype. n = 53 animals (npr-1), n = 55 animals (npr-1; malt-1(db1194)), n = 50 animals (npr-1; malt-1(syb296)), n = 29 animals (npr-1; malt-1(syb296); rab-3p::malt-1). Plots show average speed (line) and SEM (shaded regions). ***P = 2.95e−09, two-sided Mann-Whitney U test. b cDNA encoding a MALT-1 C374A catalytically inactive protein, expressed from the rab-3 promoter, does not rescue the O2 response defects of malt-1 mutants. Data corresponding to npr-1 and npr-1; malt-1; rab-3p::malt-1 in b are the same as those shown in Fig. 1m, and were obtained in parallel to the genotypes shown. n = 46 animals (npr-1), n = 74 animals (npr-1; malt-1), n = 71 animals (npr-1; malt-1; rab-3p::malt-1(C374A)), n = 46 animals (npr-1; malt-1; rab-3p::malt-1). Plots show average speed (line) and SEM (shaded regions). NS, P = 0.693918, two-sided Mann-Whitney U test. c Overexpressing MALT-1 C374A cDNA in npr-1 animals inhibits the arousal response to 21% O2. n = 53 animals (npr-1), n = 87 animals (npr-1; rab-3p::malt-1(C374A). Plots show average speed (line) and SEM (shaded regions). ***P = 1.09e−12, two-sided Mann-Whitney U test. See also Supplementary Fig. 6.