Table 1.
Dissociation constants Kd of MDM2 homodimer and MDM2-MDMX heterodimer variants for UbcH5B–Ub.
| Ligand | Kd (μM) |
|---|---|
| MDM2-350–C | 15.7 ± 0.6 |
| MDM2-350–C-6D | 6.7 ± 0.2 |
| MDM2-350–C-S386D | 13.7 ± 0.3 |
| MDM2-350–C-S395D | 13.7 ± 0.3 |
| MDM2-350–C-S407D | 13.7 ± 0.3 |
| MDM2-350–C-T419D | 13.0 ± 0.5 |
| MDM2-350–C-S425D | 13.4 ± 0.7 |
| MDM2-350–C-S429D | 7.8 ± 0.3 |
| MDM2-419–C | 14.0 ± 0.3 |
| MDM2-419–C-S429D | 7.1 ± 0.7 |
| MDM2-419–C-S429E | 6.4 ± 0.4 |
| MDM2-419–C-pS429 | 5.1 ± 0.4 |
| cat MDM2-422–C | 16.8 ± 0.3 |
| cat MDM2-422–C-S429E | 6.8 ± 0.4 |
| MDM2-350–C/MDMX-428–C | 36.7 ± 2.1 |
| MDM2-350–C-6D/MDMX-428–C | 29.2 ± 0.6 |
| MDM2-419–C/MDMX-418–C | 23.4 ± 0.8 |
| MDM2-419–C-S429E/MDMX-418–C | 17.8 ± 1.4 |
Standard error of mean values are indicated. Number of replicates, representative sensorgrams, and binding curves are shown in Supplementary Fig. 1.