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. 2020 Apr 29;10:7251. doi: 10.1038/s41598-020-64352-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Multi-step resistance selection for auranofin, clindamycin, and mupirocin evaluated against S. aureus. Drugs were serially passaged daily against (A) S. aureus ATCC 6538 and (B) methicillin-resistant S. aureus (MRSA) NRS384 (USA300) for 14 days. The minimum inhibitory concentration (MIC) of each test agent was determined after each passage. Data are presented as fold-change in MIC relative to the previous passage.