Skip to main content
. 2020 Apr 29;11(4):299. doi: 10.1038/s41419-020-2479-z

Fig. 1. KLF4 positively regulates CD9 and CD81 expression in HCC cells.

Fig. 1

a L02 and Huh7 cells were transiently transfected with KLF4-expression plasmids, then several exosome biomarkers were determined by western blots. b The regulation of CD9 and CD81 expression by KLF4 was verified by qPCR analysis after KLF4 overexpression or knockdown. c Putative KLF4 binding sites were shown in CD9 and CD81 promoters. d Wild type or Mutant CD9/CD81 promoter reporters together with KLF4 overexpression plasmid or siKLF4 were transfected into Huh7 or HCC-LM3 cells as indicated, respectively. After 24 h post transfection, the promoter activities were examined by a dual luciferase assay kit. e ChIP assays were performed using chromatins isolated from Huh7 cells transfected with Flag-tagged KLF4 plasmids. f ChIP assays were performed using endogenous KLF4 antibody in HCC-LM3 cells. One percent of the total lysates were subjected to PCR amplification before immunoprecipitation and used as Input control, and normal IgG was used as a negative control. *P < 0.05, **P < 0.05.