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. 2020 Apr 29;11(4):299. doi: 10.1038/s41419-020-2479-z

Fig. 3. CD9 and CD81 inhibit HCC cell proliferation in vitro and in vivo.

Fig. 3

a Verification of the efficiencies of CD9 and CD81 overexpression in Huh7 and Hep3B cells via western blotting. b CCK-8 assays were performed to evaluate cell proliferation rates of HCC cells described in (a), the absorbance at 450 nm was measured at the indicated time points. c Colony formation assays were employed using Huh7 and Hep3B cells transduced with LV-Vec, LV-CD9 or LV-CD81 lentiviruses, respectively. d EdU-based assays were performed to determine the effects of CD9 and CD81 on HCC cell proliferation. Representative images were shown and the percentage of EdU-positive cells were calculated and exhibited in the column graph. Scale bar = 100 μm. e Huh7 cells stably infected with LV-Vec, LV-CD9 or LV-CD81 lentiviruses were injected subcutaneously into the armpit regions of nude mice, respectively (n = 6) . After 4 weeks, the tumors were removed and weighted. The pictures of gross tumors and tumor weights were shown. *P < 0.05, **P < 0.01.