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. 2020 Apr 29;11(4):299. doi: 10.1038/s41419-020-2479-z

Fig. 4. Silenced CD9 and CD81 expression contributes to cell growth and tumorigenicity of HCC cells.

Fig. 4

a Verification the efficiencies of the siRNAs against CD9 and CD81 by western blotting in HCC-LM3 cells. b Cell growth curves indicating higher proliferative ability of HCC-LM3 cells transfected with siCD9 or siCD81 comparing with the control group. c Colony formation assays were performed after HCC-LM3 cells were stably infected with LV-NC, LV-shCD9, LV-shCD81 lentiviruses. Representative images of colonies were shown and colony number of each group was calculated. d HCC-LM3 cells described in c were implanted subcutaneously into the armpit regions of nude mice, respectively (n = 7). Tumors were removed and weighted after 4 weeks. Pictures of gross tumors and tumor weights of each group were presented. e siNC, siCD9 or siCD81 were transfected into KLF4-overexpressed HCC-LM3 cells and CCK-8 assays were performed. f Colony formation assays were performed to determine whether CD9 or CD81 mediated KLF4-induced HCC cell proliferation suppression. *P < 0.05, **P < 0.01.