Fig. 6. KLF4, CD9 or CD81 has no significant effect on HCC-derived exosome abundance.
a, b Exosomes isolated from the cell culture medium of Huh7 or HCC-LM3 cells were analyzed by TEM and NTA. Representative pictures of TEM (a) and NTA (b) were shown. c Results of NTA indicating that altered expression of KLF4, CD9 or CD81 didn’t affect the abundance of HCC-secreted exosomes. d Western blots were performed using the exosomes isolated from Huh7 cells transfected with pVEC, pKL4, pCD9 or pCD81. GM130 was used as a negative control. TEM, transmission electron microscopy. NTA, Nanoparticle tracking analysis.
