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. 2020 Apr 29;6:14. doi: 10.1038/s41523-020-0158-y

Fig. 3. Quantitative immunofluorescence (QIF) analysis of murine B7-H3 expression in normal mammary tissue and glands containing DCIS.

Fig. 3

a Representative immunofluorescence (20×, top) and H&E (10×, bottom) stained normal murine mammary tissues (left) and those containing DCIS (right). Scale bars = 200 μm. b Box plot showing quantitative IF composite scores (a.u.) of normal breast tissue (n = 5, CS = 0.01 ± 0.02 a.u.) and DCIS (n = 7, CS = 45.96 ± 12.76 a.u.). Box plot follows Tukey rules. DCIS tissues have statistically significantly higher B7-H3 staining than normal murine tissue both on the endothelial and epithelial cells (P < 0.001). c Receiver operating curve depicting the ability of murine B7-H3 expression visualized by QIF to differentiate between normal and DCIS containing tissues (AUC = 1.00, 95% CI 1.00, 1.00).