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. 2020 Apr 23;14:17. doi: 10.3389/fncir.2020.00017

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Copyright © 2020 Hanson, Swanson and Arenkiel.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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HDB GABAergic neurons preferentially synapse onto interneurons in the OB. (A) Coronal section shows the injection site with the inset showing dense membrane labeling of neurons in the HDB (dashed lines). The upper right panel shows schematically for injection of Cre-dependent Channelrhodopsin (AAV-Ef1α-flex-ChR2::eYFP) into the HDB of Vgat-Cre mice. (B) Pie charts showing proportions of GCs, M/TCs, and periglomerular cells (PGCs) responding to stimulation of ChR2-expressing HDB GABAergic terminals in OB slices. N = 11 mice total, 14–19 cells per group type. (C) Traces show an example of a light-evoked current from a granule cell (GC) responding to ChR2 stimulation (blue tick) in aCSF (black), and following serial bath application of tetrodotoxin (TTX, red), 4-aminopyridine (4AP, green), and bicuculline (BIC, purple). The image shows the corresponding biocytin cell fill spanning the GCL, mitral cell layer (MCL) and EPL. (D) The trace shows no response to light from a mitral/tufted cell (M/TC) in aCSF. The image shows the corresponding biocytin cell fill with the cell body and lateral dendrites in the MCL and apical dendrites in the GL. EPL and GCL labeled for orientation. (E) The trace shows a representative example of a light-evoked current from a PGC responding to ChR2 stimulation in aCSF and during serial additions of TTX, 4AP, and BIC. The image shows the corresponding cell fill with a cell body in and dendrites in the GL. (F) The trace shows no response to light from a non-responsive PGC in aCSF. The image shows the corresponding biocytin cell fill with the cell body and dendrites in the GL. All scale bars for traces are 5 pA and 100 ms.