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. 2020 Apr 23;14:17. doi: 10.3389/fncir.2020.00017

Figure 4.

Figure 4

Adult-born granule cells (abGCs) receive early synaptic input from HDB GABAergic neurons. (A) Schematic showing viral injections for ChR2 expression (AAV-Ef1α-flex ChR2::eYFP) and adult-born neuron birth-date labeling (lenti-CMV-tdTomato). (B) Example of birth-dated adult-born neurons in a 150 μm thick horizontal section at 14 days post-RMS injection. (C) Traces show representative examples of currents evoked by ChR2 stimulation (light blue tick) in a labeled abGC at seven DPI in aCSF (blue), and following serial bath application of tetrodotoxin (TTX, red), 4-aminopyridine (4AP, green), and bicuculline (BIC, purple). Scale bar X and Y axes are 100 ms and 10 pA. (D) Cell fill corresponding to the trace in (C) showing cell body in deep GCL and dendrite extending toward the IPL. (E) Same as (C) for abGC at 20 DPI. (F) Cell fill corresponding to the trace in (E) showing cell body in GCL and dendrite spanning IPL and EPL. (G) Amplitudes of light-evoked currents from individual birth-dated neurons at different days post RMS injection (DPI) binned by age. N = 23 mice, 60 cells. Error bars are SE. Points reflect current amplitudes (pA) from individual cells in aCSF. **p < 0.01, *p < 0.05, one-way ANOVA with Tukey’s post hoc test for multiple comparisons.