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. 2020 Apr 29;11:2095. doi: 10.1038/s41467-020-15653-7

Fig. 5. Design and characterization of a tunable NOR gate.

Fig. 5

a Schematic of all the sensor systems used (top, left), the tunable NOR gate (top, right), and their genetic implementation (bottom, dashed boxes). Yellow fluorescent protein (YFP) expression is driven by the output promoter and T1 and T3 correspond to the transcriptional terminators L3S3P11 and ECK120033737, respectively65. b Function of a two-input NOR gate. c Heatmaps showing the output of the tunable NOR gate for varying input promoter activities (Input A–PBAD: 0.008, 0.003, 0.15, 0.5, 2.5, 3.1 RPU; Input B–Ptet: 0.05, 0.5, 1.6, 3.1, 6.4, 7.5 RPU) and for low (left) and high (right) tuner promoter activities. Output promoter activities shown are average values calculated from flow cytometry data for three biological replicates. White dashed line shows an output of 1.2 RPU and denotes the transition point from a high to low output. d Flow cytometry distributions of the output YFP fluorescence for tuner promoter activities of 0.002 RPU (bottom) and 2.6 RPU (top). The four distributions correspond to combinations of the absence and presence of l-Arabinose (10 mM) and aTc (50 ng/mL). Source data are available in the Source Data file.