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. 2020 Apr 29;10:7278. doi: 10.1038/s41598-020-64308-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunofluorescence confocal microscopy showed the SIRT1 and collagen I protein expression profiles. The collagen I protein is represented by vimentin staining (red), the SIRT1 protein is represented green staining, and the nuclei are represented by DAPI staining (blue). (A) DAPI staining (a), SIRT1 (b), collagen I (c) and the merged figure (d) in the model group; DAPI staining (e), SIRT1 (f), collagen I (g) and the merged figure (h) in the control group. (B) Quantitative analysis of SIRT1 and collagen I expression in the cardiomyocytes and the epicardium. *P < 0.05.