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. 2020 Apr 29;9(4):42. doi: 10.1038/s41389-020-0226-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Representative images of tumor size in nude mice injected subcutaneously with Huh7 cells infected with either shNC or shACSL4 (n = 6, for each experimental group). The mice were killed on the 28th day for tumor weight analysis. b Tumor growth rates and tumor weight in Huh7-shNC group and Huh7-shACSL4 group. c Representative images of tumor size in nude mice injected subcutaneously with SMMC-7721 cells infected with either shNC or shACSL4. The mice were killed on the 28th day for tumor weight analysis (n = 6, for each experimental group). d Tumor growth rates and tumor weight in SMMC-7721-shNC group and SMMC-7721-shACSL4 group. The data shown are the mean ± SD of tumor volume and tumor weight from six nude mice per group (***p < 0.001). The data were analyzed using Student’s t-test. e ACSL4 and c-Myc protein levels in Huh7 indicated xenografts were detected by western blot. f H&E staining and the expression of ACSL4 and c-Myc in tumor sections by IHC analysis (scale bar: 100 μm; magnification: ×200).