Skip to main content
. 2020 Apr 23;11:656. doi: 10.3389/fimmu.2020.00656

Figure 2.

Figure 2

mSIGN-R1 (CD209b) and hDC-SIGN (CD209a) are receptors for T. gondii and play a role in invasion of T. gondii into host cells. (A) GFP labeled intracellular parasites within adherent cells (CHO vs. CHO-hDC-SIGN) and extracellular parasites after 8 h of co-culture. (A: Adherent cells on the wall of the plate; B: T. gondii infected cell; C: Extracellular parasite; D: A cluster of parasites infecting a cell); (B) Invasion assay was done to determine the invasion of T. gondii into CHO and other CHO transfectants expressing mSIGNR1 and (D) hDC-SIGN. The cells were co-cultured with GFP-labeled T. gondii for 8–18 h. The proportion of infected cells was determined by calculating the number of infected per view divided by the total number of cells per view (*P < 0.05, **P < 0.01, ***P < 0.001). (C,E) The level of internalization of T. gondii in CHO-mSIGNR1 (C) and CHO-hDC-SIGN (E) cells was calculated by determining the number of extracellular parasites per view (*P < 0.05). A total of 9 views were used to quantify the degree of internalization. Data are representative of three independent experiments.