Figure 4.

Neighbor-joining tree showing the relationship between amino acid sequences for known and select putative AH and dehydrogenase proteins. All proteins shown contain both a molybdopterin-binding domain (conserved protein domain family cd00368) and a molybdopterin-binding C-terminal (cd02775). Putative AH containing a molybdopterin-binding acetylene hydratase (cd02759) and a molybdopterin-binding acetylene hydratase C-terminal (cd02781) are indicated with green squares and known AH are indicated by red triangles. The phylogenetic affiliation of the organisms is indicated by color. The tree was constructed by downloading amino acid (aa) sequences that matched a key-word search of 'acetylene hydratase' in the NCBI GenBank database. Additional dehydrogenase sequences were included for reference. Sequences were aligned in Geneious v. 9.1.8 (Kearse et al.2012) using the ClustalW aligner then trimmed to a final length of 980 aa to compare overlapping regions of sequence. The tree was constructed using the Geneious Tree Builder with neighbor-joining methods and Jukes-Cantor distance model.