Table 3B.
Biphasic responses of PrlA/SecY membranes to inhibitor SCA-107. The IC50 (μM) inhibition of PrlA membranes were extrapolated for low IC50L and high IC50H: Ion channel activity in oocytes, and in vitro pOmpA translocation into membrane vesicles (From Hsieh et al.2017a). All suppressor strains are derivatives of MC4100 (Osborne and Silhavy 1993).
| B. Biphasic responses of PrlA mutant membranes and MIC to SecA inhibitor SCA-107 | |||||||
|---|---|---|---|---|---|---|---|
| MC4100 | PrlA4 | PrlA666 | Other PrlA, PrlG1 | ||||
| Prl membranes | IC50 | IC50L | IC50H | IC50L | IC50H | IC50L | IC50H |
| IC50 on channel activity (μM) | 3.9 | 3.4 | 125 | 1.7 | 154 | 1.4–6.1 | >100 |
| IC50 on translocation activity (μM) | 1.5 | 2.6 | >50 | 2.7 | >50 | 2.1–3.1 | >50 |
| MIC of SCA-107 (μM) | 1.5–5.5 | 3.1 | 0.8 | 1.6–3.1 | |||