Figure 3.

 LoF mutation in EPHB4. (A) HEK293T cells were transiently transfected with wild-type EPHB4 alone, mutant EPHB4 alone or the mixtures of the wild-type and mutant in the indicated ratios. (B) A375 cells were transfected with wild-type EPHB4, mutant EPHB4 or left untransfected. Cells were stimulated with either plate-bound Ephrin-B2-Fc or human IgG1 as a control. Cells were lysed, and transfected proteins were immunoprecipitated. IPs were blotted for phosphotyrosine (top) or EPHB4 (middle). Whole cell lysates were blotted for EPHB4 to demonstrate equal expression (bottom).