Figure 5.

 Perturbation attributed to ephb4a MO in developing zebrafish upregulate mTORC1 signaling and activation of mTORC1 can be inhibited by treatment of mTORC1 inhibitors. (A and B) Lysates from zebrafish larvae treated with either ephb4a MO or control MO, and untreated, or treated with Rapamycin or BEZ235 (A) or with U0126 (a MEK inhibitor) (B) were separated by SDS-PAGE and blotted for phospho-mTOR S2448, phospho-p70S6K T389 and phospho-ERKs. Blotting for β-actin was used as a loading control. Normalized quantification of p-p70S6K and p-ERKs against β-actin was performed from three independent experiments. Data are presented as mean ± SD.